Gibco™ DMEM, high glucose

Protocol tips

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	HG-DMEM supplemented with 10% FBS (Sigma-Aldrich, St. Louis, MO, USA), 50 U/mL penicillin, 50 mg/mL streptomycin, 2 mM L-glutamine (all from Life Technologies), and maintained in incubator at 37 °C in a humidified atmosphere with 5% CO2. Once they reached 80–90% of confluence, the cells were detached with 0.05% trypsin/0.2% EDTA (Sigma-Aldrich)

Protocol tips
HG-DMEM supplemented with 10% FBS (Sigma-Aldrich, St. Louis, MO, USA), 50 U/mL penicillin, 50 mg/mL streptomycin, 2 mM L-glutamine (all from Life Technologies), and maintained in incubator at 37 °C in a humidified atmosphere with 5% CO2. Once they reached 80–90% of confluence, the cells were detached with 0.05% trypsin/0.2% EDTA (Sigma-Aldrich)

Publication protocol

HG-DMEM supplemented with 10% FBS (Sigma-Aldrich, St. Louis, MO, USA), 50 U/mL penicillin, 50 mg/mL streptomycin, 2 mM L-glutamine (all from Life Technologies), and maintained in incubator at 37 °C in a humidified atmosphere with 5% CO2. Once they reached 80–90% of confluence, the cells were detached with 0.05% trypsin/0.2% EDTA (Sigma-Aldrich)

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Manufacturer protocol

Download the product protocol from Fisher Scientific for Gibco™ DMEM, high glucose below.

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