Dulbecco’s Modified Eagle’s Medium - high glucose

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	DMEM:Medium 199 (4:1) supplemented by 25 μg/ml fetuin, 5 μg/ml insulin, 0.2 μg/ml dexamethasone, 0.5 ng/ml basic fibroblast growth factor (FGF), 5 ng/ml human epidermal growth factor (EGF), 20% FBS and 1% penicillin/streptomycin

Protocol tips
DMEM:Medium 199 (4:1) supplemented by 25 μg/ml fetuin, 5 μg/ml insulin, 0.2 μg/ml dexamethasone, 0.5 ng/ml basic fibroblast growth factor (FGF), 5 ng/ml human epidermal growth factor (EGF), 20% FBS and 1% penicillin/streptomycin

Publication protocol

DMEM:Medium 199 (4:1) supplemented by 25 μg/ml fetuin, 5 μg/ml insulin, 0.2 μg/ml dexamethasone, 0.5 ng/ml basic fibroblast growth factor (FGF), 5 ng/ml human epidermal growth factor (EGF), 20% FBS and 1% penicillin/streptomycin

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Dulbecco’s Modified Eagle’s Medium - high glucose below.

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