D-MEM (High Glucose) with L-Glutamine and Phenol Red

Protocol tips

Upstream tips	Protocol tips	Downstream tips
on a 24-well tissue culture plate (ASAHI GLASS Co., Tokyo, Japan) coated with 0.1% Cellmatrix® Type IA (Nitta Gelatin Inc., Osaka, Japan)	Dulbecco's modified Eagle's medium/Nutrient mixture F12 (DMEM/F12; Wako Pure Chemical Industries, Tokyo, Japan) supplemented with 20% (v/v) fetal bovine serum (FBS; ThermoFisher Scientific KK., Yokohama, Japan), 100 ng/mL epidermal growth factor (PeproTech Inc., Rooky Hill, NJ, USA), 50 μg/mL penicillin/streptomycin, 100 μg/mL neomycin, and 0.5 μg/mL amphotericin B

Upstream tips
on a 24-well tissue culture plate (ASAHI GLASS Co., Tokyo, Japan) coated with 0.1% Cellmatrix® Type IA (Nitta Gelatin Inc., Osaka, Japan)
Protocol tips
Dulbecco's modified Eagle's medium/Nutrient mixture F12 (DMEM/F12; Wako Pure Chemical Industries, Tokyo, Japan) supplemented with 20% (v/v) fetal bovine serum (FBS; ThermoFisher Scientific KK., Yokohama, Japan), 100 ng/mL epidermal growth factor (PeproTech Inc., Rooky Hill, NJ, USA), 50 μg/mL penicillin/streptomycin, 100 μg/mL neomycin, and 0.5 μg/mL amphotericin B

Publication protocol

Dulbecco's modified Eagle's medium/Nutrient mixture F12 (DMEM/F12; Wako Pure Chemical Industries, Tokyo, Japan) supplemented with 20% (v/v) fetal bovine serum (FBS; ThermoFisher Scientific KK., Yokohama, Japan), 100 ng/mL epidermal growth factor (PeproTech Inc., Rooky Hill, NJ, USA), 50 μg/mL penicillin/streptomycin, 100 μg/mL neomycin, and 0.5 μg/mL amphotericin B

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Manufacturer protocol

Download the product protocol from Fujifilm Wako Chemicals Europe Gmbh for D-MEM (High Glucose) with L-Glutamine and Phenol Red below.

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