Iscove’s Modified Dulbecco’s Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	IMDM (Biochrome, Merck, Berlin, Germany) supplemented with growth factors: 50 ng/ml stem cell factor (SCF), 50 ng/ml TPO, 1 ng/ml IL-3 and 20 ng/ml IL-6. 4 days later the growth factor mix was changed to 50 ng/ml TPO and 10 ng/ml IL-1β as described previously by Heideveld et al. (all from PeproTech, Pittsburgh, USA)

Protocol tips
IMDM (Biochrome, Merck, Berlin, Germany) supplemented with growth factors: 50 ng/ml stem cell factor (SCF), 50 ng/ml TPO, 1 ng/ml IL-3 and 20 ng/ml IL-6. 4 days later the growth factor mix was changed to 50 ng/ml TPO and 10 ng/ml IL-1β as described previously by Heideveld et al. (all from PeproTech, Pittsburgh, USA)

Publication protocol

IMDM (Biochrome, Merck, Berlin, Germany) supplemented with growth factors: 50 ng/ml stem cell factor (SCF), 50 ng/ml TPO, 1 ng/ml IL-3 and 20 ng/ml IL-6. 4 days later the growth factor mix was changed to 50 ng/ml TPO and 10 ng/ml IL-1β as described previously by Heideveld et al. (all from PeproTech, Pittsburgh, USA)

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Iscove’s Modified Dulbecco’s Medium below.

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