ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool

Protocol tips

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	A431 cells were all purchased from the ATCC and cultured in DMEM containing 10% FBS, 1% penicillin/streptomycin, and 1% glutamine at 37 °C and 5% CO2. Knockdown in A431 cells were achieved by transfection of siRNA using the AMAXA nucleofection method, solution V, and protocols X-001. The following siRNA oligos were used: control siRNA 1, 5’-GCAACGGCAUUCCACCUUU(TT)-3’; control siRNA 2 control pool of four siRNAs (Dharmacon, catalog no. D001810-1020); RCP (SMARTpool of four siRNAs (Dharmacon, catalog no. L-015968-00-0005) was used. All siRNA oligos were used as a combination of two or four siRNAs, and each individual siRNA was tested for efficiency of knockdown and off-target effects.

Protocol tips

A431 cells were all purchased from the ATCC and cultured in DMEM containing 10% FBS, 1% penicillin/streptomycin, and 1% glutamine at 37 °C and 5% CO2. Knockdown in A431 cells were achieved by transfection of siRNA using the AMAXA nucleofection method, solution V, and protocols X-001. The following siRNA oligos were used: control siRNA 1, 5’-GCAACGGCAUUCCACCUUU(TT)-3’; control siRNA 2 control pool of four siRNAs (Dharmacon, catalog no. D001810-1020); RCP (SMARTpool of four siRNAs (Dharmacon, catalog no. L-015968-00-0005) was used. All siRNA oligos were used as a combination of two or four siRNAs, and each individual siRNA was tested for efficiency of knockdown and off-target effects.

Publication protocol

A431 cells were all purchased from the ATCC and cultured in DMEM containing 10% FBS, 1% penicillin/streptomycin, and 1% glutamine at 37 °C and 5% CO2. Knockdown in A431 cells were achieved by transfection of siRNA using the AMAXA nucleofection method, solution V, and protocols X-001. The following siRNA oligos were used: control siRNA 1, 5’-GCAACGGCAUUCCACCUUU(TT)-3’; control siRNA 2 control pool of four siRNAs (Dharmacon, catalog no. D001810-1020); RCP (SMARTpool of four siRNAs (Dharmacon, catalog no. L-015968-00-0005) was used. All siRNA oligos were used as a combination of two or four siRNAs, and each individual siRNA was tested for efficiency of knockdown and off-target effects.

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Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool below.

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