VEGF-D siRNA (h)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The RCC cell line Caki-2, was obtained from American Type Culture Collection. All cultures were maintained according to the manufacturer’s suggestions. VEGF-D siRNA (catalog no. sc-39844), and control scramble siRNA (catalog no. sc-37007) were purchased from Santa Cruz Biotechnology. A total of 2 * 105 cells were plated in a six-well plate for 24 h, and siRNA transfection was conducted using the Lipofectamine 2000 kit according to the manufacturer’s instructions (Invitrogen).

Protocol tips

The RCC cell line Caki-2, was obtained from American Type Culture Collection. All cultures were maintained according to the manufacturer’s suggestions. VEGF-D siRNA (catalog no. sc-39844), and control scramble siRNA (catalog no. sc-37007) were purchased from Santa Cruz Biotechnology. A total of 2 * 105 cells were plated in a six-well plate for 24 h, and siRNA transfection was conducted using the Lipofectamine 2000 kit according to the manufacturer’s instructions (Invitrogen).

Publication protocol

The RCC cell line Caki-2, was obtained from American Type Culture Collection. All cultures were maintained according to the manufacturer’s suggestions. VEGF-D siRNA (catalog no. sc-39844), and control scramble siRNA (catalog no. sc-37007) were purchased from Santa Cruz Biotechnology. A total of 2 * 105 cells were plated in a six-well plate for 24 h, and siRNA transfection was conducted using the Lipofectamine 2000 kit according to the manufacturer’s instructions (Invitrogen).

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Manufacturer protocol

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