FlexiTube GeneSolution GS7052 for TGM2

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	Human primary RCC cell lines Caki-2 was obtained from the American Type Culture Collection. Caki-2 was maintained in McCoy’s 5A modified medium, which contained 10% (v/ v) Fetal bovine serum (FBS) and penicillin/streptomycin (100 units/ml and 100 mg/ml, respectively). For in vitro gene knockdown experiments, non-silencing control (NS) siRNA (AllStars SI03650318) human 148 Y. BAGATUR ET AL. TGM2 targeting siRNAs (Hs_TGM2_1:SI00743715 and Hs_TGM2_6:SI03055465) were purchased from Qiagen. Transfection was performed using RNAiFact transfection reagent (Qiagen) according to the manufacture’s protocol, as described previous

Protocol tips

Human primary RCC cell lines Caki-2 was obtained from the American Type Culture Collection. Caki-2 was maintained in McCoy’s 5A modified medium, which contained 10% (v/ v) Fetal bovine serum (FBS) and penicillin/streptomycin (100 units/ml and 100 mg/ml, respectively). For in vitro gene knockdown experiments, non-silencing control (NS) siRNA (AllStars SI03650318) human 148 Y. BAGATUR ET AL. TGM2 targeting siRNAs (Hs_TGM2_1:SI00743715 and Hs_TGM2_6:SI03055465) were purchased from Qiagen. Transfection was performed using RNAiFact transfection reagent (Qiagen) according to the manufacture’s protocol, as described previous

Publication protocol

Human primary RCC cell lines Caki-2 was obtained from the American Type Culture Collection. Caki-2 was maintained in McCoy’s 5A modified medium, which contained 10% (v/ v) Fetal bovine serum (FBS) and penicillin/streptomycin (100 units/ml and 100 mg/ml, respectively). For in vitro gene knockdown experiments, non-silencing control (NS) siRNA (AllStars SI03650318) human 148 Y. BAGATUR ET AL. TGM2 targeting siRNAs (Hs_TGM2_1:SI00743715 and Hs_TGM2_6:SI03055465) were purchased from Qiagen. Transfection was performed using RNAiFact transfection reagent (Qiagen) according to the manufacture’s protocol, as described previous

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Manufacturer protocol

Download the product protocol from Qiagen for FlexiTube GeneSolution GS7052 for TGM2 below.

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