RCAS1 siRNA (h)

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	ES2 ovarian cancer cells (gifts from S. Eblen and C. Schweinfest, Medical University of South Carolina) were maintained at 37°C with 5% CO2 in McCoy's 5A (ES2) supplemented with L ‐glutamine, 10% fetal bovine serum (FBS, Valley Biomedical Inc, Winchester, VA, USA) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA). Serum starved medium was supplemented with 0.1% FBS and 1% penicillin/streptomycin. RNAi directed against RCAS1 (Santa Cruz, Santa Cruz, CA) was transiently transfected using Lipofectamine (Invitrogen) by procedures outlined by the manufacturer. Cells were assessed 48 hr post transfection.

Protocol tips

ES2 ovarian cancer cells (gifts from S. Eblen and C. Schweinfest, Medical University of South Carolina) were maintained at 37°C with 5% CO2 in McCoy's 5A (ES2) supplemented with L ‐glutamine, 10% fetal bovine serum (FBS, Valley Biomedical Inc, Winchester, VA, USA) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA). Serum starved medium was supplemented with 0.1% FBS and 1% penicillin/streptomycin. RNAi directed against RCAS1 (Santa Cruz, Santa Cruz, CA) was transiently transfected using Lipofectamine (Invitrogen) by procedures outlined by the manufacturer. Cells were assessed 48 hr post transfection.

Publication protocol

ES2 ovarian cancer cells (gifts from S. Eblen and C. Schweinfest, Medical University of South Carolina) were maintained at 37°C with 5% CO2 in McCoy's 5A (ES2) supplemented with L ‐glutamine, 10% fetal bovine serum (FBS, Valley Biomedical Inc, Winchester, VA, USA) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA). Serum starved medium was supplemented with 0.1% FBS and 1% penicillin/streptomycin. RNAi directed against RCAS1 (Santa Cruz, Santa Cruz, CA) was transiently transfected using Lipofectamine (Invitrogen) by procedures outlined by the manufacturer. Cells were assessed 48 hr post transfection.

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Manufacturer protocol

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