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A2780 cell was purchased from Sigma. siRNA for MOAP-1 was purchased from Dharmacon (Lafayette, CO, USA; Cat. no. L-004430-00). siRNA transfection was performed following the manufacturer’s protocol of Lipofectamine RNAiMAX Transfection Reagent. In all, 20 nM (final concentration) of siRNA was transfected twice in a 24-h interval. |
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Protocol tips |
A2780 cell was purchased from Sigma. siRNA for MOAP-1 was purchased from Dharmacon (Lafayette, CO, USA; Cat. no. L-004430-00). siRNA transfection was performed following the manufacturer’s protocol of Lipofectamine RNAiMAX Transfection Reagent. In all, 20 nM (final concentration) of siRNA was transfected twice in a 24-h interval. |
Publication protocol
A2780 cell was purchased from Sigma. siRNA for MOAP-1 was purchased from Dharmacon (Lafayette, CO, USA; Cat. no. L-004430-00). siRNA transfection was performed following the manufacturer’s protocol of Lipofectamine RNAiMAX Transfection Reagent. In all, 20 nM (final concentration) of siRNA was transfected twice in a 24-h interval.
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