PE Annexin V Apoptosis Detection Kit I

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells treated with sorafenib (0–4 μM) alone or in combination with other agent.	- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Upstream tips
- Cells treated with sorafenib (0–4 μM) alone or in combination with other agent.
Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Publication protocol

Cells were seeded onto six wells and treated with sorafenib (0–4 μM) alone or in combination as described above. After 48 h, the cells were harvested and centrifuged. Apoptosis was measured by using the PE Annexin V Apoptosis Detection Kit I in combination with flow cytometry (Becton Dickinson, Schwechat, Austria) according to the manufacturer's protocols. Assays for caspase 3/7 activity were performed with the Caspase-Glo 3/7 assay kit (Promega) according to the manufacturer's protocols (Santer et al. 2011).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Apoptosis assay cell type - PC-3 using PE Annexin V Apoptosis Detection Kit I from BD Biosciences.

Manufacturer protocol

Download the product protocol from BD Biosciences for PE Annexin V Apoptosis Detection Kit I below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Apoptosis assay cell type - PC-3 using PE Annexin V Apoptosis Detection Kit I from BD Biosciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.