ON-TARGETplus Human BECN1 siRNA

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	The human glioblastoma cell line U251 was a gift from Dr. Jong Yun (Penn State College of Medicine). All cell culture media were supplemented with 10% fetal bovine serum, 100 units/ml penicillin, and 100 μg/ml streptomycin. Cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2/95% air. Cells were cultured in six‐well plates at 30–40% confluence on the day of transfection. Control or Beclin 1 (Dharmacon, J‐010552‐05) siRNA at final concentration of 17 nM was mixed with 2.5 μl RNAi MAX (Invitrogen) for each well, according to the manufacturer's protocol. After 72 h of transfection, cells were collected for further experiments.

Protocol tips

The human glioblastoma cell line U251 was a gift from Dr. Jong Yun (Penn State College of Medicine). All cell culture media were supplemented with 10% fetal bovine serum, 100 units/ml penicillin, and 100 μg/ml streptomycin. Cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2/95% air. Cells were cultured in six‐well plates at 30–40% confluence on the day of transfection. Control or Beclin 1 (Dharmacon, J‐010552‐05) siRNA at final concentration of 17 nM was mixed with 2.5 μl RNAi MAX (Invitrogen) for each well, according to the manufacturer's protocol. After 72 h of transfection, cells were collected for further experiments.

Publication protocol

The human glioblastoma cell line U251 was a gift from Dr. Jong Yun (Penn State College of Medicine). All cell culture media were supplemented with 10% fetal bovine serum, 100 units/ml penicillin, and 100 μg/ml streptomycin. Cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2/95% air. Cells were cultured in six‐well plates at 30–40% confluence on the day of transfection. Control or Beclin 1 (Dharmacon, J‐010552‐05) siRNA at final concentration of 17 nM was mixed with 2.5 μl RNAi MAX (Invitrogen) for each well, according to the manufacturer's protocol. After 72 h of transfection, cells were collected for further experiments.

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