siGENOME Human TBX2 (6909) siRNA - SMARTpool

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	U251 cell lines were obtained from American Type Culture Collection (Manassas, VA, USA). The cells were cultured in Dulbecco’s Modified Eagle’s Medium (Invitrogen, Carlsbad, CA, USA) containing 10% fetal calf serum (Invitrogen). siGENOME Tbx2 siRNAs pool and negative siRNAs pool were obtained from Dharmacon (GE Healthcare, Piscataway, NJ, USA). siRNA was transfected with DharmaFECT1 (GE Healthcare). For each well of a 6-well plate, 0.2 nmol siRNA was used for transfection.

Protocol tips

U251 cell lines were obtained from American Type Culture Collection (Manassas, VA, USA). The cells were cultured in Dulbecco’s Modified Eagle’s Medium (Invitrogen, Carlsbad, CA, USA) containing 10% fetal calf serum (Invitrogen). siGENOME Tbx2 siRNAs pool and negative siRNAs pool were obtained from Dharmacon (GE Healthcare, Piscataway, NJ, USA). siRNA was transfected with DharmaFECT1 (GE Healthcare). For each well of a 6-well plate, 0.2 nmol siRNA was used for transfection.

Publication protocol

U251 cell lines were obtained from American Type Culture Collection (Manassas, VA, USA). The cells were cultured in Dulbecco’s Modified Eagle’s Medium (Invitrogen, Carlsbad, CA, USA) containing 10% fetal calf serum (Invitrogen). siGENOME Tbx2 siRNAs pool and negative siRNAs pool were obtained from Dharmacon (GE Healthcare, Piscataway, NJ, USA). siRNA was transfected with DharmaFECT1 (GE Healthcare). For each well of a 6-well plate, 0.2 nmol siRNA was used for transfection.

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