siGENOME Human IKBKB (3551) siRNA - SMARTpool

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	Panc-1 (CRL-1496) and pancreatic cancer cell lines was obtained from the American Type Culture Collection (Manassas, VA). Panc-1 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum and 100 units/ml penicillin/streptomycin. Cells were cultured in DMEM supplemented with 0.5% fetal bovine serum for 24 hour prior to experimentation. All cell culture reagents were obtained from Invitrogen (Carlsbad, CA). The following siRNA (siGenome SMARTpool) was obtained from Dharmacon (Layfayette, CO) as a pool of four annealed double-stranded RNA oligonucleotides: IKKβ (M-003503-03), and non-targeting control #3 (D001201-03). In brief, cells were cultured to 70% confluency in six-well plates. Dharmafect 1 transfection reagent (Layfayette, CO) was used to transfect 100nM siRNA according to manufacturer’s instruction.

Protocol tips

Panc-1 (CRL-1496) and pancreatic cancer cell lines was obtained from the American Type Culture Collection (Manassas, VA). Panc-1 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum and 100 units/ml penicillin/streptomycin. Cells were cultured in DMEM supplemented with 0.5% fetal bovine serum for 24 hour prior to experimentation. All cell culture reagents were obtained from Invitrogen (Carlsbad, CA). The following siRNA (siGenome SMARTpool) was obtained from Dharmacon (Layfayette, CO) as a pool of four annealed double-stranded RNA oligonucleotides: IKKβ (M-003503-03), and non-targeting control #3 (D001201-03). In brief, cells were cultured to 70% confluency in six-well plates. Dharmafect 1 transfection reagent (Layfayette, CO) was used to transfect 100nM siRNA according to manufacturer’s instruction.

Publication protocol

Panc-1 (CRL-1496) and pancreatic cancer cell lines was obtained from the American Type Culture Collection (Manassas, VA). Panc-1 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum and 100 units/ml penicillin/streptomycin. Cells were cultured in DMEM supplemented with 0.5% fetal bovine serum for 24 hour prior to experimentation. All cell culture reagents were obtained from Invitrogen (Carlsbad, CA). The following siRNA (siGenome SMARTpool) was obtained from Dharmacon (Layfayette, CO) as a pool of four annealed double-stranded RNA oligonucleotides: IKKβ (M-003503-03), and non-targeting control #3 (D001201-03). In brief, cells were cultured to 70% confluency in six-well plates. Dharmafect 1 transfection reagent (Layfayette, CO) was used to transfect 100nM siRNA according to manufacturer’s instruction.

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