Human Apoptosis Array G1

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1.0 x 106 cells were seeded.	- Incubate arrays with sample at 40C overnight. - Incubation may be done for 2 hours at room temperature.	- avoid solution flowing into neighboring wells for better reading

Upstream tips
- 1.0 x 106 cells were seeded.
Protocol tips
- Incubate arrays with sample at 40C overnight. - Incubation may be done for 2 hours at room temperature.
Downstream tips
- avoid solution flowing into neighboring wells for better reading

Publication protocol

A total of 1.0 x 106 cells were untreated and treated for 6 h. The cell lysates were normalized using BCA Protein Assay kit (Pierce, USA) and incubated at 4°C overnight with Raybio® Human Apoptosis Antibody Array slides (RayBiotech Inc. USA, GA). All slides were washed and a cocktail of biotinylated antibody mix was used to detect apoptosis-related proteins. After incubation with Hylate Plus™-conjugated streptavidin, signals were detected with a fluorescence scanner by Axon Genepix® using the Cy2 channel. Shortlisted protein expression target were selected based on a fold change threshold of ≥ 1.5 or ≤ -1.5.

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Manufacturer protocol

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