ON-TARGETplus Human SAMHD1 (25939) siRNA - SMARTpool

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	The human skin fibroblast cell line HFF1 (ATCC® SCRC-1041™) cells were cultured in DMEM, supplemented with 15% and 10%, respectively. Cells were transiently transfected with an 80 nM final concentration of small interfering RNAs (siRNAs) targeting SAMHD1 by using Lipofectamine® 2000 (Thermo Fisher Scientific). siRNA (ON-TARGETplus SMARTpool) used in this study were from Dharmacon, SAMHD1 (L-013950-01) and a nontargeting pool (NT) was used as a negative control.

Protocol tips

The human skin fibroblast cell line HFF1 (ATCC® SCRC-1041™) cells were cultured in DMEM, supplemented with 15% and 10%, respectively. Cells were transiently transfected with an 80 nM final concentration of small interfering RNAs (siRNAs) targeting SAMHD1 by using Lipofectamine® 2000 (Thermo Fisher Scientific). siRNA (ON-TARGETplus SMARTpool) used in this study were from Dharmacon, SAMHD1 (L-013950-01) and a nontargeting pool (NT) was used as a negative control.

Publication protocol

The human skin fibroblast cell line HFF1 (ATCC® SCRC-1041™) cells were cultured in DMEM, supplemented with 15% and 10%, respectively. Cells were transiently transfected with an 80 nM final concentration of small interfering RNAs (siRNAs) targeting SAMHD1 by using Lipofectamine® 2000 (Thermo Fisher Scientific). siRNA (ON-TARGETplus SMARTpool) used in this study were from Dharmacon, SAMHD1 (L-013950-01) and a nontargeting pool (NT) was used as a negative control.

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