Hs_IFI16_1 FlexiTube siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Human CD34+ TF-1 cells, was grown in RPMI1640 media, and HeLa cells in D-MEM media, all supplemented with 10% heatinactivated foetal bovine serum (FBS). TF-1 cells were grown in the presence of 2ng/ml recombinant human GM-CSF (R&D Systems, Abingdon, UK).Transient transfections were performed using the Amaxa Nucleofector system (program T-003). For IFI16 knockdown studies, 0.5–2μg of IFI16 siRNA (code: SI00445676; Qiagen, Crawley, UK) or control siRNA (code: 1027280) were co-transfected with pcDNA4-N1ICD or empty vector. Efficiency of siRNA knockdown was assessed by FACS analysis and RT-PCR.

Protocol tips

Human CD34+ TF-1 cells, was grown in RPMI1640 media, and HeLa cells in D-MEM media, all supplemented with 10% heatinactivated foetal bovine serum (FBS). TF-1 cells were grown in the presence of 2ng/ml recombinant human GM-CSF (R&D Systems, Abingdon, UK).Transient transfections were performed using the Amaxa Nucleofector system (program T-003). For IFI16 knockdown studies, 0.5–2μg of IFI16 siRNA (code: SI00445676; Qiagen, Crawley, UK) or control siRNA (code: 1027280) were co-transfected with pcDNA4-N1ICD or empty vector. Efficiency of siRNA knockdown was assessed by FACS analysis and RT-PCR.

Publication protocol

Human CD34+ TF-1 cells, was grown in RPMI1640 media, and HeLa cells in D-MEM media, all supplemented with 10% heatinactivated foetal bovine serum (FBS). TF-1 cells were grown in the presence of 2ng/ml recombinant human GM-CSF (R&D Systems, Abingdon, UK).Transient transfections were performed using the Amaxa Nucleofector system (program T-003). For IFI16 knockdown studies, 0.5–2μg of IFI16 siRNA (code: SI00445676; Qiagen, Crawley, UK) or control siRNA (code: 1027280) were co-transfected with pcDNA4-N1ICD or empty vector. Efficiency of siRNA knockdown was assessed by FACS analysis and RT-PCR.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - TF‐1 IFI16 using Hs_IFI16_1 FlexiTube siRNA from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for Hs_IFI16_1 FlexiTube siRNA below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - TF‐1 IFI16 using Hs_IFI16_1 FlexiTube siRNA from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.