Annexin V-FITC Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	After incubation for 48 h, the cells were washed with PBS, trypinsized, and stained with fluorescein isothycanateconjugated annexin V using Annexin V-FITC kit (Miltenyi Biotec, Germany) and propidium iodide (PI), following the manufacturer’s protocol.

Protocol tips
After incubation for 48 h, the cells were washed with PBS, trypinsized, and stained with fluorescein isothycanateconjugated annexin V using Annexin V-FITC kit (Miltenyi Biotec, Germany) and propidium iodide (PI), following the manufacturer’s protocol.

Publication protocol

Apoptosis assay with flow cytometry analysis. 0.5 mL of
Ovcar-8 cells (300 000 cells/mL) was seeded in 12 well
plates for 24 h and exposed to medium as control,
carboplatin, cycleanine, and tetrandrine (20 μM). After
incubation for 48 h, the cells were washed with PBS,
trypinsized, and stained with fluorescein isothycanateconjugated annexin V using Annexin V-FITC kit
(Miltenyi Biotec, Germany) and propidium iodide
(PI), following the manufacturer’s protocol. Apoptotic
and live cells were measured using a Beckman Coulter
Cytomics 500 flow cytometer with CXP software (High
Wycombe UK). Flow cytometry data were analyzed
using FLOWING SOFTWARE (Perttu Terho, Turku Centre
for Biotechnology, University of Turku, Finland). The
percentage of early and later apoptotic and live cells in
three independent experiments was statistically analyzed using GRAPHPAD PRISM 6 software.

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Manufacturer protocol

Download the product protocol from Miltenyibiotec for Annexin V-FITC Kit below.

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