Hs_KEAP1_5 FlexiTube siRNA

Protocol tips

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	Transfection of cDNA was performed by using Lipofectamine Plus (Invitrogen), and HiPerfect was used for transfection of small interfering RNA (siRNA); both were used according to the manufacturer’s instructions. p62 siRNA (SI00057596) and Keap1 siRNA (SI03246439) were purchased from Qiagen. Cells were transfected with wild-type Keap1 (Keap1- WT) and Keap1-C151S, along with plasmids encoding mouse glutathione S-transferase (mGST)–ARE–firefly luciferase and thymidine kinase (TK)- Renilla luciferase (internal control). Luciferase activities were measured by using the Promega dual-luciferase reporter assay system. The experiment was run in triplicate and repeated three times. Results are expressed as means  standard deviations (SD). A P value of 0.05 was considered to be significant

Protocol tips

Transfection of cDNA was performed by using Lipofectamine Plus (Invitrogen), and HiPerfect was used for transfection of small interfering RNA (siRNA); both were used according to the manufacturer’s instructions. p62 siRNA (SI00057596) and Keap1 siRNA (SI03246439) were purchased from Qiagen. Cells were transfected with wild-type Keap1 (Keap1- WT) and Keap1-C151S, along with plasmids encoding mouse glutathione S-transferase (mGST)–ARE–firefly luciferase and thymidine kinase (TK)- Renilla luciferase (internal control). Luciferase activities were measured by using the Promega dual-luciferase reporter assay system. The experiment was run in triplicate and repeated three times. Results are expressed as means  standard deviations (SD). A P value of 0.05 was considered to be significant

Publication protocol

Transfection of cDNA was performed by using Lipofectamine Plus (Invitrogen), and HiPerfect was used for transfection of small interfering RNA (siRNA); both were used according to the manufacturer’s instructions. p62 siRNA (SI00057596) and Keap1 siRNA (SI03246439) were purchased from Qiagen. Cells were transfected with wild-type Keap1 (Keap1- WT) and Keap1-C151S, along with plasmids encoding mouse glutathione S-transferase (mGST)–ARE–firefly luciferase and thymidine kinase (TK)- Renilla luciferase (internal control). Luciferase activities were measured by using the Promega dual-luciferase reporter assay system. The experiment was run in triplicate and repeated three times. Results are expressed as means  standard deviations (SD). A P value of 0.05 was considered to be significant

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Manufacturer protocol

Download the product protocol from Qiagen for Hs_KEAP1_5 FlexiTube siRNA below.

Download manufacturer protocol

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