ON-TARGETplus Human CDK1 (983) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	HEK293T cells were seeded in 12-well plates (3×105/well) at 24 h before transfection. Cells were transfected with 100 nM of SMARTpool ON-TARGET plus siRNA specific for CDK1 (L003224), CDK2 (L003236), or a scramble control (Dharmacon) using Trans-IT TKO (Mirus) according to manufacturer’s protocol and two rounds of transfection were performed as described (St Gelais et al., 2014).

Protocol tips
HEK293T cells were seeded in 12-well plates (3×105/well) at 24 h before transfection. Cells were transfected with 100 nM of SMARTpool ON-TARGET plus siRNA specific for CDK1 (L003224), CDK2 (L003236), or a scramble control (Dharmacon) using Trans-IT TKO (Mirus) according to manufacturer’s protocol and two rounds of transfection were performed as described (St Gelais et al., 2014).

Publication protocol

HEK293T cells were seeded in 12-well plates (3×105/well) at 24 h before transfection. Cells were transfected with 100 nM of SMARTpool ON-TARGET plus siRNA specific for CDK1 (L003224), CDK2 (L003236), or a scramble control (Dharmacon) using Trans-IT TKO (Mirus) according to manufacturer’s protocol and two rounds of transfection were performed as described (St Gelais et al., 2014)

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - HEK293 CDK1 using ON-TARGETplus Human CDK1 (983) siRNA - SMARTpool from Horizon Discovery Ltd..

Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Human CDK1 (983) siRNA - SMARTpool below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - HEK293 CDK1 using ON-TARGETplus Human CDK1 (983) siRNA - SMARTpool from Horizon Discovery Ltd.. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.