siGENOME Human PDCD1LG2 (80380) siRNA - SMARTpool

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	PD-L2 antibody specificity checking was performed on the two cell lines (M244 and M381), which were seeded on a six-well plate (target confluency of ∼80% on the day of flow cytometry analysis) on day 1. Cells were transfected with 25 nM of a PD-L2 siRNA (GE Dharmacon, SMARTpool: siGENOME PDCD1LG2 siRNA and non-targeting control siRNA) as per the manufacturer’s protocol on day 2. On day 3, the selected groups were exposed to 100 IU/mL interferon gamma, and flow cytometry analyses were performed on day 4 as described above.

Protocol tips

PD-L2 antibody specificity checking was performed on the two cell lines (M244 and M381), which were seeded on a six-well plate (target confluency of ∼80% on the day of flow cytometry analysis) on day 1. Cells were transfected with 25 nM of a PD-L2 siRNA (GE Dharmacon, SMARTpool: siGENOME PDCD1LG2 siRNA and non-targeting control siRNA) as per the manufacturer’s protocol on day 2. On day 3, the selected groups were exposed to 100 IU/mL interferon gamma, and flow cytometry analyses were performed on day 4 as described above.

Publication protocol

PD-L2 antibody specificity checking was performed on the two cell lines (M244 and M381), which were seeded on a six-well plate (target confluency of ∼80% on the day of flow cytometry analysis) on day 1. Cells were transfected with 25 nM of a PD-L2 siRNA (GE Dharmacon, SMARTpool: siGENOME PDCD1LG2 siRNA and non-targeting control siRNA) as per the manufacturer’s protocol on day 2. On day 3, the selected groups were exposed to 100 IU/mL interferon gamma, and flow cytometry analyses were performed on day 4 as described above.

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Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for siGENOME Human PDCD1LG2 (80380) siRNA - SMARTpool below.

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