ON-TARGETplus Human ERCC4 (2072) siRNA - SMARTpool

Protocol tips

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	U2OS cells were transfected with the indicated small interfering RNAs (siRNAs),U2OS cells were re-seeded into 6 well plates at a density of 300 cells/ well 72 hours post-siRNA transfection. RNAi transfections were performed using either Dharmafect 1 (ThermoFisher) or Lipofectamine RNAiMAX (Invitrogen) in a forward transfection mode following the manufacturers protocols. 5 hours after transfection, the medium was substituted for fresh medium. Cells were generally collected 24 hours after plasmid transfection and 72 hours after RNAi transfection.

Protocol tips

U2OS cells were transfected with the indicated small interfering RNAs (siRNAs),U2OS cells were re-seeded into 6 well plates at a density of 300 cells/ well 72 hours post-siRNA transfection. RNAi transfections were performed using either Dharmafect 1 (ThermoFisher) or Lipofectamine RNAiMAX (Invitrogen) in a forward transfection mode following the manufacturers protocols. 5 hours after transfection, the medium was substituted for fresh medium. Cells were generally collected 24 hours after plasmid transfection and 72 hours after RNAi transfection.

Publication protocol

U2OS cells were transfected with the indicated small interfering RNAs (siRNAs),U2OS cells were re-seeded into 6 well plates at a density of 300 cells/ well 72 hours post-siRNA transfection. RNAi transfections were performed using either Dharmafect 1 (ThermoFisher) or Lipofectamine RNAiMAX (Invitrogen) in a forward transfection mode following the manufacturers protocols. 5 hours after transfection, the medium was substituted for fresh medium. Cells were generally collected 24 hours after plasmid transfection and 72 hours after RNAi transfection.

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Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Human ERCC4 (2072) siRNA - SMARTpool below.

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