siGENOME TRIM43 siRNA

Protocol tips

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	HEK 293 cells (all purchased from ATCC) as well as Huh7 (provided by M. Farzan, Scripps Institute Florida), were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; Thermo Fisher Scientific), 2 mM GlutaMAX (Thermo Fisher Scientific), and 1% (v/v) penicillin-streptomycin (Thermo Fisher Scientific) under standard culture conditions.HEK 293T cells were seeded into 24- or 12-well plates (~ 2.5 or 5 × 105 cells per well). The next day, cells were transfected with 60 nM or 120 nM of SiRNAs targeting TRIM43 (siGENOME SMARTpool M-007127-01-0050), using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s instructions. At 48 h post-transfection, knockdown efficiency of endogenous TRIM proteins was determined by qRT-PCR or immunoblot (IB) analysis, as described below.

Protocol tips

HEK 293 cells (all purchased from ATCC) as well as Huh7 (provided by M. Farzan, Scripps Institute Florida), were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; Thermo Fisher Scientific), 2 mM GlutaMAX (Thermo Fisher Scientific), and 1% (v/v) penicillin-streptomycin (Thermo Fisher Scientific) under standard culture conditions.HEK 293T cells were seeded into 24- or 12-well plates (~ 2.5 or 5 × 105 cells per well). The next day, cells were transfected with 60 nM or 120 nM of SiRNAs targeting TRIM43 (siGENOME SMARTpool M-007127-01-0050), using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s instructions. At 48 h post-transfection, knockdown efficiency of endogenous TRIM proteins was determined by qRT-PCR or immunoblot (IB) analysis, as described below.

Publication protocol

HEK 293 cells (all purchased from ATCC) as well as Huh7 (provided by M. Farzan, Scripps Institute Florida), were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; Thermo Fisher Scientific), 2 mM GlutaMAX (Thermo Fisher Scientific), and 1% (v/v) penicillin-streptomycin (Thermo Fisher Scientific) under standard culture conditions.HEK 293T cells were seeded into 24- or 12-well plates (~ 2.5 or 5 × 105 cells per well). The next day, cells were transfected with 60 nM or 120 nM of SiRNAs targeting TRIM43 (siGENOME SMARTpool M-007127-01-0050), using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s instructions. At 48 h post-transfection, knockdown efficiency of endogenous TRIM proteins was determined by qRT-PCR or immunoblot (IB) analysis, as described below.

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