RAB8A siRNA

Protocol tips

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	"HEK293T cells were seeded in 6-well plates at 30–40% confluence one day prior to transfection such that they were at a confluence of 70–80% the following day. They were transfected with 50 nM siRNA using 4 μl of jetPRIME Transfection Reagent (Polyplus-Transfection SA, no 114–15) in 200 μl jetPRIME buffer."

Protocol tips
"HEK293T cells were seeded in 6-well plates at 30–40% confluence one day prior to transfection such that they were at a confluence of 70–80% the following day. They were transfected with 50 nM siRNA using 4 μl of jetPRIME Transfection Reagent (Polyplus-Transfection SA, no 114–15) in 200 μl jetPRIME buffer."

Publication protocol

"HEK293T cells were seeded in 6-well plates at 30–40% confluence one day prior to transfection such that they were at a confluence of 70–80% the following day. They were transfected with 50 nM siRNA using 4 μl of jetPRIME Transfection Reagent (Polyplus-Transfection SA, no 114–15) in 200 μl jetPRIME buffer."

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RAB8A siRNA below.

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