Cell Death Detection ELISAPLUS

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 10,000 cells per well were platted for an assay - Cells were pre treted with ABC294640, paclitaxel, or vehicle for 24 h.		- When the absorbance of samples too high,reduce agent-concentration and decrease incubation period. - When the absorbance of samples too low, increase the agent concentration and prolong the agent incubation interval

Upstream tips
- 10,000 cells per well were platted for an assay - Cells were pre treted with ABC294640, paclitaxel, or vehicle for 24 h.
Downstream tips
- When the absorbance of samples too high,reduce agent-concentration and decrease incubation period. - When the absorbance of samples too low, increase the agent concentration and prolong the agent incubation interval

Publication protocol

Caov-3 cells were plated at 10,000 cells per well in DMEM and allowed to adhere overnight. Cells were then treated with ABC294640, paclitaxel, or vehicle for 24 h. A Cell Death Detection ELISA PLUS Kit from Roche Applied Science (Indianapolis, IN, USA) was used to process cells. Briefly, cells were lysed and transferred to an anti-nucleosome-coated microplate, incubated with colorimetric detection reagent, and the absorbance was read on a MicroTek plate reader at 405 nm.

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Manufacturer protocol

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