Stealth siRNA™ NOTCH1

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	The THP-1 (acute monoblastic leukemia) cell line was supplied by the Health Science Research Resources Bank (Osaka, Japan),The cells were cultured in 10% fetal calf serum (FCS)-supplemented RPMI-1640 medium at 37˚C in a humidified 5% CO2 atmosphere .pre-designed siRNAs (Stealth siRNA™)targeting NOTCH1 (siN1: HSS107248, 107249, and 181550) was purchased from Life Technologies (Carlsbad, CA, USA). The stealth RNAi negative control Duplex was used as a control siRNA. Each siRNA was transfected at a concentration of 40 nM using the pipette tip chamber-based electroporation, the Neon™ Transfection System (Life Technologies), according to the manufacturer’s instructions. After transfection, the cells were quickly transferred into culture medium.

Protocol tips

The THP-1 (acute monoblastic leukemia) cell line was supplied by the Health Science Research Resources Bank (Osaka, Japan),The cells were cultured in 10% fetal calf serum (FCS)-supplemented RPMI-1640 medium at 37˚C in a humidified 5% CO2 atmosphere .pre-designed siRNAs (Stealth siRNA™)targeting NOTCH1 (siN1: HSS107248, 107249, and 181550) was purchased from Life Technologies (Carlsbad, CA, USA). The stealth RNAi negative control Duplex was used as a control siRNA. Each siRNA was transfected at a concentration of 40 nM using the pipette tip chamber-based electroporation, the Neon™ Transfection System (Life Technologies), according to the manufacturer’s instructions. After transfection, the cells were quickly transferred into culture medium.

Publication protocol

The THP-1 (acute monoblastic leukemia) cell line was supplied by the Health Science Research Resources Bank (Osaka, Japan),The cells were cultured in 10% fetal calf serum (FCS)-supplemented RPMI-1640 medium at 37˚C in a humidified 5% CO2 atmosphere .pre-designed siRNAs (Stealth siRNA™)targeting NOTCH1 (siN1: HSS107248, 107249, and 181550) was purchased from Life Technologies (Carlsbad, CA, USA). The stealth RNAi negative control Duplex was used as a control siRNA. Each siRNA was transfected at a concentration of 40 nM using the pipette tip chamber-based electroporation, the Neon™ Transfection System (Life Technologies), according to the manufacturer’s instructions. After transfection, the cells were quickly transferred into culture medium.

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Manufacturer protocol

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