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For gene silencing, cells were transiently transfected with siRNA SMARTpool reagents purchased from Thermo Scientific Dharmacon (Lafayette, CO, USA) using Oligofectamine (Invitrogen) according to the manufacturer’s instructions. SMARTPool siRNAs used were: siRNA FOXM1 (L-009762-00), siRNA NBS1 (L-009641-00) and the NS control siRNA, confirmed to have minimal targeting of known genes (D-001810-10-05). |
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Protocol tips |
For gene silencing, cells were transiently transfected with siRNA SMARTpool reagents purchased from Thermo Scientific Dharmacon (Lafayette, CO, USA) using Oligofectamine (Invitrogen) according to the manufacturer’s instructions. SMARTPool siRNAs used were: siRNA FOXM1 (L-009762-00), siRNA NBS1 (L-009641-00) and the NS control siRNA, confirmed to have minimal targeting of known genes (D-001810-10-05). |
Publication protocol
For gene silencing, cells were transiently transfected with siRNA SMARTpool reagents purchased from Thermo Scientific Dharmacon (Lafayette, CO, USA) using Oligofectamine (Invitrogen) according to the manufacturer’s instructions. SMARTPool siRNAs used were: siRNA FOXM1 (L-009762-00), siRNA NBS1 (L-009641-00) and the NS control siRNA, confirmed to have minimal targeting of known genes (D-001810-10-05).
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Manufacturer protocol
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