ON-TARGETplus Human WEE1 (7465) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Cells were plated in 6-well plates at 2.0 ×105 cells per well in RPMI complete media. The following day the media was replaced with 1ml Opti-MEM (Invitrogen) and the cells were transfected with 0.5ml complexes of Lipofectamine 2000 (0.5%, Invitrogen) and siRNA for either Mcl-1, BIM, BAK, Wee1 or non-targeting controls (40nM, Dharmacon On-Target Plus pools). Medium was replaced after 3–6 hours with 5% FBS in RPMI, and the cells were analyzed 120 hr after transfection. In the BIM and BAK studies, 300nM XL888 was also added 48 hours after transfection.

Protocol tips

Cells were plated in 6-well plates at 2.0 ×105 cells per well in RPMI complete media. The following day the media was replaced with 1ml Opti-MEM (Invitrogen) and the cells were transfected with 0.5ml complexes of Lipofectamine 2000 (0.5%, Invitrogen) and siRNA for either Mcl-1, BIM, BAK, Wee1 or non-targeting controls (40nM, Dharmacon On-Target Plus pools). Medium was replaced after 3–6 hours with 5% FBS in RPMI, and the cells were analyzed 120 hr after transfection. In the BIM and BAK studies, 300nM XL888 was also added 48 hours after transfection.

Publication protocol

Cells were plated in 6-well plates at 2.0 ×105 cells per well in RPMI complete media. The following day the media was replaced with 1ml Opti-MEM (Invitrogen) and the cells were transfected with 0.5ml complexes of Lipofectamine 2000 (0.5%, Invitrogen) and siRNA for either Mcl-1, BIM, BAK, Wee1 or non-targeting controls (40nM, Dharmacon On-Target Plus pools). Medium was replaced after 3–6 hours with 5% FBS in RPMI, and the cells were analyzed 120 hr after transfection. In the BIM and BAK studies, 300nM XL888 was also added 48 hours after transfection.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - M245 WEE1 using ON-TARGETplus Human WEE1 (7465) siRNA - SMARTpool from Horizon Discovery Ltd..

Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Human WEE1 (7465) siRNA - SMARTpool below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - M245 WEE1 using ON-TARGETplus Human WEE1 (7465) siRNA - SMARTpool from Horizon Discovery Ltd.. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.