siGENOME Human ARAP1 (116985) siRNA - SMARTpool

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	siRNA oligonucleotides were purchased from Dharmacon [catalogue number M‐009136‐02 (Smart pool) and MU‐009136‐02 (set of four Upgrade siRNA duplexes) for human ARAP1, LOC361617 for rat ARAP1 and D‐001810‐10 for ON‐TARGETplus siCONTROL non‐targeting pool] and transfected using Oligofectamine (Invitrogen), according to the manufacturer’s instructions. The four oligonucleotides present in the pool that were also tested separately were 1 (5′‐GAGAAGGAGUGGCCUAUUA‐3′), 2 (5′‐GCAGGGAUCUUACAUCUAU‐3′), 3 (5′‐GGAGAUCACUGCCAUUGUG‐3′) and 4 (5′‐ACAAGAAUCUAGAGGAGUA‐3′).A significant reduction in ARAP1 levels was obtained after 72 h siRNAs treatment in HeLa cells (80 ± 5%) and after 48 h in NRK cells (70 ± 7%). All the experiments reported were performed in HeLa cells using the Smart pool of the four oligonucleotides and then repeated with at least two different single oligonucleotides (mainly 1 and 4).

Protocol tips

siRNA oligonucleotides were purchased from Dharmacon [catalogue number M‐009136‐02 (Smart pool) and MU‐009136‐02 (set of four Upgrade siRNA duplexes) for human ARAP1, LOC361617 for rat ARAP1 and D‐001810‐10 for ON‐TARGETplus siCONTROL non‐targeting pool] and transfected using Oligofectamine (Invitrogen), according to the manufacturer’s instructions. The four oligonucleotides present in the pool that were also tested separately were 1 (5′‐GAGAAGGAGUGGCCUAUUA‐3′), 2 (5′‐GCAGGGAUCUUACAUCUAU‐3′), 3 (5′‐GGAGAUCACUGCCAUUGUG‐3′) and 4 (5′‐ACAAGAAUCUAGAGGAGUA‐3′).A significant reduction in ARAP1 levels was obtained after 72 h siRNAs treatment in HeLa cells (80 ± 5%) and after 48 h in NRK cells (70 ± 7%). All the experiments reported were performed in HeLa cells using the Smart pool of the four oligonucleotides and then repeated with at least two different single oligonucleotides (mainly 1 and 4).

Publication protocol

siRNA oligonucleotides were purchased from Dharmacon [catalogue number M‐009136‐02 (Smart pool) and MU‐009136‐02 (set of four Upgrade siRNA duplexes) for human ARAP1, LOC361617 for rat ARAP1 and D‐001810‐10 for ON‐TARGETplus siCONTROL non‐targeting pool] and transfected using Oligofectamine (Invitrogen), according to the manufacturer’s instructions. The four oligonucleotides present in the pool that were also tested separately were 1 (5′‐GAGAAGGAGUGGCCUAUUA‐3′), 2 (5′‐GCAGGGAUCUUACAUCUAU‐3′), 3 (5′‐GGAGAUCACUGCCAUUGUG‐3′) and 4 (5′‐ACAAGAAUCUAGAGGAGUA‐3′).A significant reduction in ARAP1 levels was obtained after 72 h siRNAs treatment in HeLa cells (80 ± 5%) and after 48 h in NRK cells (70 ± 7%). All the experiments reported were performed in HeLa cells using the Smart pool of the four oligonucleotides and then repeated with at least two different single oligonucleotides (mainly 1 and 4).

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