ON-TARGETplus Human EGFR (1956) siRNA - SMARTpool

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	The small inhibitor duplex RNAs (siRNAs) (ON-target plus SMARTpool), siRET (human: # L-003170-00), siEGFR (human: # L-003114-00), and siKDR (human: # L-003148-00) were from Dharmacon (Lafayette, CO, USA). The siCONTROL nontargeting pool (# D-001206-13-05) was used as a negative control. Cells were transfected with 100 nM siRNAs using Dharmafect reagent following the manufacturer's instructions. The day before transfection, the cells were plated on 35 mm dishes at 40% of confluence in RPMI supplemented with 4% fetal bovine serum (FBS) without antibiotics. Cells were harvested 48 h after the transfection.

Protocol tips

The small inhibitor duplex RNAs (siRNAs) (ON-target plus SMARTpool), siRET (human: # L-003170-00), siEGFR (human: # L-003114-00), and siKDR (human: # L-003148-00) were from Dharmacon (Lafayette, CO, USA). The siCONTROL nontargeting pool (# D-001206-13-05) was used as a negative control. Cells were transfected with 100 nM siRNAs using Dharmafect reagent following the manufacturer's instructions. The day before transfection, the cells were plated on 35 mm dishes at 40% of confluence in RPMI supplemented with 4% fetal bovine serum (FBS) without antibiotics. Cells were harvested 48 h after the transfection.

Publication protocol

The small inhibitor duplex RNAs (siRNAs) (ON-target plus SMARTpool), siRET (human: # L-003170-00), siEGFR (human: # L-003114-00), and siKDR (human: # L-003148-00) were from Dharmacon (Lafayette, CO, USA). The siCONTROL nontargeting pool (# D-001206-13-05) was used as a negative control. Cells were transfected with 100 nM siRNAs using Dharmafect reagent following the manufacturer's instructions. The day before transfection, the cells were plated on 35 mm dishes at 40% of confluence in RPMI supplemented with 4% fetal bovine serum (FBS) without antibiotics. Cells were harvested 48 h after the transfection.

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