Arp2 siRNA (h)

Protocol tips

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	Synthetic siRNAs targeting ERα (siRNA SMARTpool ESR1), ERβ (siRNA SMARTpool ESR2), c-SRC (siRNA SMARTpool SRC), FAK (siRNA SMARTpool FAK), and control siRNAs (D-001810–01-05) were purchased from Dharmacon (Lafayette, CO). Gβ1 siRNAs, N-WASP, and Arp-2 siRNAs were from Santa Cruz Biotechnology (Santa Cruz, CA). The siRNAs were used at the final concentration of 50 nM. Breast cancer cells were treated 48 h after siRNAs transfection. Efficacy of gene silencing was checked with Western analysis and found to be optimal at 48 h.

Protocol tips

Synthetic siRNAs targeting ERα (siRNA SMARTpool ESR1), ERβ (siRNA SMARTpool ESR2), c-SRC (siRNA SMARTpool SRC), FAK (siRNA SMARTpool FAK), and control siRNAs (D-001810–01-05) were purchased from Dharmacon (Lafayette, CO). Gβ1 siRNAs, N-WASP, and Arp-2 siRNAs were from Santa Cruz Biotechnology (Santa Cruz, CA). The siRNAs were used at the final concentration of 50 nM. Breast cancer cells were treated 48 h after siRNAs transfection. Efficacy of gene silencing was checked with Western analysis and found to be optimal at 48 h.

Publication protocol

Synthetic siRNAs targeting ERα (siRNA SMARTpool ESR1), ERβ (siRNA SMARTpool ESR2), c-SRC (siRNA SMARTpool SRC), FAK (siRNA SMARTpool FAK), and control siRNAs (D-001810–01-05) were purchased from Dharmacon (Lafayette, CO). Gβ1 siRNAs, N-WASP, and Arp-2 siRNAs were from Santa Cruz Biotechnology (Santa Cruz, CA). The siRNAs were used at the final concentration of 50 nM. Breast cancer cells were treated 48 h after siRNAs transfection. Efficacy of gene silencing was checked with Western analysis and found to be optimal at 48 h.

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Manufacturer protocol

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