Annexin V / 7-AAD

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells were exposed to 250, 500 and 1000 µM of compound 3a and 10, 25 or 50 µM of 3b and 4 for 24 h		- The incubation with Annexin A5-FITC and 7-AAD should be carried out on ice so as to arrest further progress of the cells through the stages of viability > apoptosis > secondary necrosis.

Upstream tips
- Cells were exposed to 250, 500 and 1000 µM of compound 3a and 10, 25 or 50 µM of 3b and 4 for 24 h
Downstream tips
- The incubation with Annexin A5-FITC and 7-AAD should be carried out on ice so as to arrest further progress of the cells through the stages of viability > apoptosis > secondary necrosis.

Publication protocol

The assay was performed according to the manufacturer’s instructions using Annexin V-PE and 7-AAD (Beckman Coulter, Marseille, Cedex 9, France) kits. Briefly, MCF-7 and HepG2 cells were exposed to 250, 500 and 1000 µM of compound 3a and 10, 25 or 50 µM of 3b and 4 for 24 h. The amount of apoptosis/necrosis in the treated HepG2 and MCF-7 cells was assessed by flow cytometry using the detailed reported protocol [38].

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Manufacturer protocol

Download the product protocol from Beckman Coulter for Annexin V / 7-AAD below.

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