Stealth siRNA_SPI1

Protocol tips

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	PU.1 siRNA (Stealth Select RNAi, HSS144058, HSS144060, and HSS186060), GATA1 siRNA (HSS142150, HSS142151, and HSS142152), GATA2 siRNA (HSS104003, HSS104004, HSS178122), c-kit siRNA (HSS105821), and control siRNA (Stealth RNAi Negative Universal Control Lo GC, Med GC, and Hi GC [12935-200, 300, and 400]) were purchased from Invitrogen (Carlsbad, CA). Transfection of siRNA was performed, as described previously (14). Briefly, 10 (or 1) ml 20 mmol/L siRNA was introduced into 2 3 106 (or 2 3 105) LAD2 cells or primary mast cells with a Neon 100 ml kit (or a Neon 10 ml kit) using a Neon transfection system (Invitrogen) set at Program 16 (for LAD2) or 5 (for primary mast cells).

Protocol tips

PU.1 siRNA (Stealth Select RNAi, HSS144058, HSS144060, and HSS186060), GATA1 siRNA (HSS142150, HSS142151, and HSS142152), GATA2 siRNA (HSS104003, HSS104004, HSS178122), c-kit siRNA (HSS105821), and control siRNA (Stealth RNAi Negative Universal Control Lo GC, Med GC, and Hi GC [12935-200, 300, and 400]) were purchased from Invitrogen (Carlsbad, CA). Transfection of siRNA was performed, as described previously (14). Briefly, 10 (or 1) ml 20 mmol/L siRNA was introduced into 2 3 106 (or 2 3 105) LAD2 cells or primary mast cells with a Neon 100 ml kit (or a Neon 10 ml kit) using a Neon transfection system (Invitrogen) set at Program 16 (for LAD2) or 5 (for primary mast cells).

Publication protocol

PU.1 siRNA (Stealth Select RNAi, HSS144058, HSS144060, and HSS186060), GATA1 siRNA (HSS142150, HSS142151, and HSS142152), GATA2 siRNA (HSS104003, HSS104004, HSS178122), c-kit siRNA (HSS105821), and control siRNA (Stealth RNAi Negative Universal Control Lo GC, Med GC, and Hi GC [12935-200, 300, and 400]) were purchased from Invitrogen (Carlsbad, CA). Transfection of siRNA was performed, as described previously (14). Briefly, 10 (or 1) ml 20 mmol/L siRNA was introduced into 2 3 106 (or 2 3 105) LAD2 cells or primary mast cells with a Neon 100 ml kit (or a Neon 10 ml kit) using a Neon transfection system (Invitrogen) set at Program 16 (for LAD2) or 5 (for primary mast cells).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Stealth siRNA_SPI1 below.

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