ON-TARGETplus Human NCR3LG1 (374383) siRNA - SMARTpool

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	To target B7H6 expression in tumor cell lines, a mix of four siRNAs complementary to human B7H6 (ON‐TARGETplus SMARTpool, Dharmacon, Thermo Fisher Scientific) was used. In a 24‐well plate, 3.6 × 105 cells were plated in each well. After 24 h, 7.2 pmol siRNA (B7H6 or control) was mixed with 1.2 μL RNAiMAX (Thermo Fisher Scientific) (both dissolved in OptiMEM), incubated at room temperature for 20 min and added to the cells in 500 μL of complete medium. 60–66 h after transfection start, cells were washed twice in OptiMEM, and complete medium was added. Cells were harvested for reporter assays 72 h after transfection start. Surface expression of B7H6 was analyzed by flow cytometry at 72 and 96 h after transfection start.

Protocol tips

To target B7H6 expression in tumor cell lines, a mix of four siRNAs complementary to human B7H6 (ON‐TARGETplus SMARTpool, Dharmacon, Thermo Fisher Scientific) was used. In a 24‐well plate, 3.6 × 105 cells were plated in each well. After 24 h, 7.2 pmol siRNA (B7H6 or control) was mixed with 1.2 μL RNAiMAX (Thermo Fisher Scientific) (both dissolved in OptiMEM), incubated at room temperature for 20 min and added to the cells in 500 μL of complete medium. 60–66 h after transfection start, cells were washed twice in OptiMEM, and complete medium was added. Cells were harvested for reporter assays 72 h after transfection start. Surface expression of B7H6 was analyzed by flow cytometry at 72 and 96 h after transfection start.

Publication protocol

To target B7H6 expression in tumor cell lines, a mix of four siRNAs complementary to human B7H6 (ON‐TARGETplus SMARTpool, Dharmacon, Thermo Fisher Scientific) was used. In a 24‐well plate, 3.6 × 105 cells were plated in each well. After 24 h, 7.2 pmol siRNA (B7H6 or control) was mixed with 1.2 μL RNAiMAX (Thermo Fisher Scientific) (both dissolved in OptiMEM), incubated at room temperature for 20 min and added to the cells in 500 μL of complete medium. 60–66 h after transfection start, cells were washed twice in OptiMEM, and complete medium was added. Cells were harvested for reporter assays 72 h after transfection start. Surface expression of B7H6 was analyzed by flow cytometry at 72 and 96 h after transfection start.

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Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Human NCR3LG1 (374383) siRNA - SMARTpool below.

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