siGENOME Human ATG7 (10533) siRNA - SMARTpool

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	A pool of four RNAi duplexes (smartpool) targeting human RIP3 (M-003534-01-0005), TSC2 (M-003029-03-0005), MK2 (M-003516-02-0005) and ATG7 (M-020112-01-0005) were purchased from Dharmacon. RNAi against human YWHAZ was designed with the BLOCK-iT™ RNAi Designer tools (Invitrogen) (5′-AGTTCTTGATCCCCAATGC-3′). Control RNAi was purchased from Applied Biosystems. RNAis were used at a concentration of 50 nM and transfection performed using calcium phosphate (ProFection® Mammalian Transfection System, Promega).

Protocol tips

A pool of four RNAi duplexes (smartpool) targeting human RIP3 (M-003534-01-0005), TSC2 (M-003029-03-0005), MK2 (M-003516-02-0005) and ATG7 (M-020112-01-0005) were purchased from Dharmacon. RNAi against human YWHAZ was designed with the BLOCK-iT™ RNAi Designer tools (Invitrogen) (5′-AGTTCTTGATCCCCAATGC-3′). Control RNAi was purchased from Applied Biosystems. RNAis were used at a concentration of 50 nM and transfection performed using calcium phosphate (ProFection® Mammalian Transfection System, Promega).

Publication protocol

A pool of four RNAi duplexes (smartpool) targeting human RIP3 (M-003534-01-0005), TSC2 (M-003029-03-0005), MK2 (M-003516-02-0005) and ATG7 (M-020112-01-0005) were purchased from Dharmacon. RNAi against human YWHAZ was designed with the BLOCK-iT™ RNAi Designer tools (Invitrogen) (5′-AGTTCTTGATCCCCAATGC-3′). Control RNAi was purchased from Applied Biosystems. RNAis were used at a concentration of 50 nM and transfection performed using calcium phosphate (ProFection® Mammalian Transfection System, Promega).

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