Annexin V-FITC Apoptosis Kit

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	The cell apoptosis was measured by flow cytometry analysis with an Annexin-V/FITC and propidium iodide (PI) apoptosis detection kit (BioVision, USA), according to the manufacturer’s standards. Briefly, after 48 h of transfection, the cells were collected and suspended in annexin-binding buffer and exposed to Annexin-VFITC and PI for 15 min in the dark at room temperature. The apoptotic percentage of cells was quantified by flow cytometry

Protocol tips

The cell apoptosis was measured by flow cytometry analysis with an Annexin-V/FITC and propidium iodide (PI) apoptosis detection kit (BioVision, USA), according to the manufacturer’s standards. Briefly, after 48 h of transfection, the cells were collected and suspended in annexin-binding buffer and exposed to Annexin-VFITC and PI for 15 min in the dark at room temperature. The apoptotic percentage of cells was quantified by flow cytometry

Publication protocol

The cell apoptosis was measured by flow cytometry
analysis with an Annexin-V/FITC and propidium iodide
(PI) apoptosis detection kit (BioVision, USA), according to the manufacturer’s standards. Briefly, after 48 h
of transfection, the cells were collected and suspended
in annexin-binding buffer and exposed to Annexin-VFITC and PI for 15 min in the dark at room temperature. The apoptotic percentage of cells was quantified by
flow cytometry

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