ON-TARGET plus Mouse Becn1 (56208) siRNA - SMARTpool

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	4T1 cells were obtained from ATCC. Both cell lines were maintained in DMEM (Cellgro, 10-013-CV) + 10% fetal bovine serum (FBS, Sigma, F6178) at 37C and 5% CO24T1 cells were transfected with Dharmacon BECN-1 ON-TARGETplus SMARTpool siRNAs (L-055895-00) or with ON-TARGETplus non-targeting siRNA #1 (D-001810-02-20) using Dharmafect-2 transfection reagent (Thermo Fisher Scientific, T-2002-03). Twenty-four hours after transfection, cells were trypsinized and plated for experiments. Protein knockdown was evaluated 72 h after transfection.

Protocol tips

4T1 cells were obtained from ATCC. Both cell lines were maintained in DMEM (Cellgro, 10-013-CV) + 10% fetal bovine serum (FBS, Sigma, F6178) at 37C and 5% CO24T1 cells were transfected with Dharmacon BECN-1 ON-TARGETplus SMARTpool siRNAs (L-055895-00) or with ON-TARGETplus non-targeting siRNA #1 (D-001810-02-20) using Dharmafect-2 transfection reagent (Thermo Fisher Scientific, T-2002-03). Twenty-four hours after transfection, cells were trypsinized and plated for experiments. Protein knockdown was evaluated 72 h after transfection.

Publication protocol

4T1 cells were obtained from ATCC. Both cell lines were maintained in DMEM (Cellgro, 10-013-CV) + 10% fetal bovine serum (FBS, Sigma, F6178) at 37C and 5% CO24T1 cells were transfected with Dharmacon BECN-1 ON-TARGETplus SMARTpool siRNAs (L-055895-00) or with ON-TARGETplus non-targeting siRNA #1 (D-001810-02-20) using Dharmafect-2 transfection reagent (Thermo Fisher Scientific, T-2002-03). Twenty-four hours after transfection, cells were trypsinized and plated for experiments. Protein knockdown was evaluated 72 h after transfection.

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