FlexiTube GeneSolution GS27279 for Tnfrsf12a

siRNA / miRNA gene silencing Mouse - B16-BL6 FN14/Tnfrsf12a

Experiment
siRNA / miRNA gene silencing Mouse - B16-BL6 FN14/Tnfrsf12a
Product
FlexiTube GeneSolution GS27279 for Tnfrsf12a from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
The parental murine B16-BL6 melanoma cell line [57, 58] was provided by Dr. Sarah Netzel-Arnett (University of Maryland School of Medicine) and maintained in DMEM (Corning) supplemented with 10% FBS (Sigma-Aldrich), 2 mM L-glutamine and 1% penicillin-streptomycin (both from Corning). All cells were maintained at 37°C in 5% CO2. Cells were detached with non-enzymatic cell dissociation solution (Sigma-Aldrich) for routine passaging and experiments.Cells were plated, allowed to attach for 5 hr, and then transfected using Lipofectamine RNAiMax transfection reagent (Invitrogen) according to the manufacturers’ instructions with either negative control siRNA, Fn14 siRNA #1 or #4 targeted to the mouse Fn14 transcript (all three siRNAs at a final concentration of 20 nM),. All siRNAs were purchased from Qiagen: negative control (#1022076), mouse Fn14 (#SI01452311, #SI01452332). Cells were harvested at 24 hr post-transfection and seeded onto Matrigel invasion chambers as described above, with the remaining cells harvested at either 24 or 48 hr post-transfection for Western blot analysis.

Publication protocol

The parental murine B16-BL6 melanoma cell line [57, 58] was provided by Dr. Sarah Netzel-Arnett (University of Maryland School of Medicine) and maintained in DMEM (Corning) supplemented with 10% FBS (Sigma-Aldrich), 2 mM L-glutamine and 1% penicillin-streptomycin (both from Corning). All cells were maintained at 37°C in 5% CO2. Cells were detached with non-enzymatic cell dissociation solution (Sigma-Aldrich) for routine passaging and experiments.Cells were plated, allowed to attach for 5 hr, and then transfected using Lipofectamine RNAiMax transfection reagent (Invitrogen) according to the manufacturers’ instructions with either negative control siRNA, Fn14 siRNA #1 or #4 targeted to the mouse Fn14 transcript (all three siRNAs at a final concentration of 20 nM),. All siRNAs were purchased from Qiagen: negative control (#1022076), mouse Fn14 (#SI01452311, #SI01452332). Cells were harvested at 24 hr post-transfection and seeded onto Matrigel invasion chambers as described above, with the remaining cells harvested at either 24 or 48 hr post-transfection for Western blot analysis.

Full paper   Login or join for free to view the full paper.

Reviews

FlexiTube GeneSolution GS27279 for Tnfrsf12a from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Mouse - B16-BL6 FN14/Tnfrsf12a using FlexiTube GeneSolution GS27279 for Tnfrsf12a from Qiagen.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for FlexiTube GeneSolution GS27279 for Tnfrsf12a below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Mouse - B16-BL6 FN14/Tnfrsf12a using FlexiTube GeneSolution GS27279 for Tnfrsf12a from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms