ON-TARGETplus Mouse Casp8 (12370) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	All cell lines were purchased from American Type Culture Collection (Manassas, VA, USA). Mouse colon adenocarcinoma CT26 cell lines were cultured in RPMI 1640 (Gibco) supplemented with 10% heat-inactivated FBS (PAA), 2 mM GlutaMAX I CTS (Gibco) and 100 U/mL penicillin + 100 µg/mL streptomycin (Gibco). A total of 1 × 105 CT26 cells per well were plated in six-well plates and allowed to reach 50% confluence on the day of transfection. siRNA specific for mouse caspase-8 (ON-TARGET plus Mouse Casp8 siRNA SMART pool L-043044-00-0010) and an unrelated control (ON-TARGET plus Non-targeting pool D-001810-10-05) were purchased from Dharmacon. Cells were transfected with siRNA at a final concentration of 25 nM using Lipofectamine® RNAiMAX Transfection Reagent (Invitrogen) according to the manufacturer's instructions. Knockdown of caspase-8 was confirmed by western blotting.

Protocol tips

All cell lines were purchased from American Type Culture Collection (Manassas, VA, USA). Mouse colon adenocarcinoma CT26 cell lines were cultured in RPMI 1640 (Gibco) supplemented with 10% heat-inactivated FBS (PAA), 2 mM GlutaMAX I CTS (Gibco) and 100 U/mL penicillin + 100 µg/mL streptomycin (Gibco). A total of 1 × 105 CT26 cells per well were plated in six-well plates and allowed to reach 50% confluence on the day of transfection. siRNA specific for mouse caspase-8 (ON-TARGET plus Mouse Casp8 siRNA SMART pool L-043044-00-0010) and an unrelated control (ON-TARGET plus Non-targeting pool D-001810-10-05) were purchased from Dharmacon. Cells were transfected with siRNA at a final concentration of 25 nM using Lipofectamine® RNAiMAX Transfection Reagent (Invitrogen) according to the manufacturer's instructions. Knockdown of caspase-8 was confirmed by western blotting.

Publication protocol

All cell lines were purchased from American Type Culture Collection (Manassas, VA, USA). Mouse colon adenocarcinoma CT26 cell lines were cultured in RPMI 1640 (Gibco) supplemented with 10% heat-inactivated FBS (PAA), 2 mM GlutaMAX I CTS (Gibco) and 100 U/mL penicillin + 100 µg/mL streptomycin (Gibco). A total of 1 × 105 CT26 cells per well were plated in six-well plates and allowed to reach 50% confluence on the day of transfection. siRNA specific for mouse caspase-8 (ON-TARGET plus Mouse Casp8 siRNA SMART pool L-043044-00-0010) and an unrelated control (ON-TARGET plus Non-targeting pool D-001810-10-05) were purchased from Dharmacon. Cells were transfected with siRNA at a final concentration of 25 nM using Lipofectamine® RNAiMAX Transfection Reagent (Invitrogen) according to the manufacturer's instructions. Knockdown of caspase-8 was confirmed by western blotting.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Mouse - CT26 caspase-8 using ON-TARGETplus Mouse Casp8 (12370) siRNA - SMARTpool from Horizon Discovery Ltd..

Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Mouse Casp8 (12370) siRNA - SMARTpool below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Mouse - CT26 caspase-8 using ON-TARGETplus Mouse Casp8 (12370) siRNA - SMARTpool from Horizon Discovery Ltd.. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.