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"A mixture of double-stranded RNA nucleotides targeting four different sequences of mouse Myb mRNA (ON-TARGETplus SMARTpool L-044112-00-0005) and a non-targeting negative control siRNA were obtained from Dharmacon (Thermo Fisher Scientific., Lafayette, CO). NIH3T3 cells were transfected with siRNA
oligonucleotide pools using DharmaFECT reagent according to the manufacturer’s protocol (Dharmacon)." |
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Protocol tips |
"A mixture of double-stranded RNA nucleotides targeting four different sequences of mouse Myb mRNA (ON-TARGETplus SMARTpool L-044112-00-0005) and a non-targeting negative control siRNA were obtained from Dharmacon (Thermo Fisher Scientific., Lafayette, CO). NIH3T3 cells were transfected with siRNA
oligonucleotide pools using DharmaFECT reagent according to the manufacturer’s protocol (Dharmacon)." |
Publication protocol
A mixture of double-stranded RNA nucleotides targeting four different sequences of mouse Myb mRNA (ON-TARGETplus SMARTpool L-044112-00-0005) and a non-targeting negative control siRNA were obtained from Dharmacon (Thermo Fisher Scientific., Lafayette, CO). NIH3T3 cells were transfected with siRNA
oligonucleotide pools using DharmaFECT reagent according to the manufacturer’s protocol (Dharmacon
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