ON-TARGETplus Mouse Cflar (12633) siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	SiRNAs against caspase-8 (5′-GUGAAUGGAACCUGGUAUA-3′ and 5′-GUCACGGACUUCAGACAAA-3′, catalogue number J-043044-05 and J-043044-06, respectively) and FLIP (5′-GGAGCAAGAUCAAAUAUGA-3′ and 5′-GAAUAGACUUGAACACAAA-3′, catalogue number J-041091-05 and J-041091-08 respectively) as well as scramble siRNA control (5′-UGGUUUACAUGUCGACUAA-3′, catalogue number D-001810-01) were ordered from Dharmacon, and introduced into cells using Lipofectamine RNAiMAX (Invitrogen) according to manufacturer’s guidelines.

Protocol tips

SiRNAs against caspase-8 (5′-GUGAAUGGAACCUGGUAUA-3′ and 5′-GUCACGGACUUCAGACAAA-3′, catalogue number J-043044-05 and J-043044-06, respectively) and FLIP (5′-GGAGCAAGAUCAAAUAUGA-3′ and 5′-GAAUAGACUUGAACACAAA-3′, catalogue number J-041091-05 and J-041091-08 respectively) as well as scramble siRNA control (5′-UGGUUUACAUGUCGACUAA-3′, catalogue number D-001810-01) were ordered from Dharmacon, and introduced into cells using Lipofectamine RNAiMAX (Invitrogen) according to manufacturer’s guidelines.

Publication protocol

SiRNAs against caspase-8 (5′-GUGAAUGGAACCUGGUAUA-3′ and 5′-GUCACGGACUUCAGACAAA-3′, catalogue number J-043044-05 and J-043044-06, respectively) and FLIP (5′-GGAGCAAGAUCAAAUAUGA-3′ and 5′-GAAUAGACUUGAACACAAA-3′, catalogue number J-041091-05 and J-041091-08 respectively) as well as scramble siRNA control (5′-UGGUUUACAUGUCGACUAA-3′, catalogue number D-001810-01) were ordered from Dharmacon, and introduced into cells using Lipofectamine RNAiMAX (Invitrogen) according to manufacturer’s guidelines

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Papers

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Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Mouse Cflar (12633) siRNA below.

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