ON-TARGETplus Mouse Zbp1 (58203) siRNA - SMARTpool

Protocol tips

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	FLAG-tagged WT and mutRLR-A DAI (Kaiser et al., 2008) were cloned into the pQCXIH retroviral vector (Clontech). FLAG-tagged WT and mRHIM RIP3 retroviral and RIP3-A (TRCN0000022535), RIP3-B (TRCN0000022538), and control lentiviral shRNA constructs were previously described (Upton et al., 2010). Retro- and lentiviral production, infection, and selection were previously described (Kaiser et al., 2008). siRNA transfections were performed with 200 pg of nontargeting, DAI, or RIP3 ON-TARGETplus SMARTpool siRNAs (Dharmacon) and Lipofectamine RNAiMAXX (Invitrogen) according to the manufacturer's recommendations, and assays were performed 48 hr posttransfection

Protocol tips

FLAG-tagged WT and mutRLR-A DAI (Kaiser et al., 2008) were cloned into the pQCXIH retroviral vector (Clontech). FLAG-tagged WT and mRHIM RIP3 retroviral and RIP3-A (TRCN0000022535), RIP3-B (TRCN0000022538), and control lentiviral shRNA constructs were previously described (Upton et al., 2010). Retro- and lentiviral production, infection, and selection were previously described (Kaiser et al., 2008). siRNA transfections were performed with 200 pg of nontargeting, DAI, or RIP3 ON-TARGETplus SMARTpool siRNAs (Dharmacon) and Lipofectamine RNAiMAXX (Invitrogen) according to the manufacturer's recommendations, and assays were performed 48 hr posttransfection

Publication protocol

FLAG-tagged WT and mutRLR-A DAI (Kaiser et al., 2008) were cloned into the pQCXIH retroviral vector (Clontech). FLAG-tagged WT and mRHIM RIP3 retroviral and RIP3-A (TRCN0000022535), RIP3-B (TRCN0000022538), and control lentiviral shRNA constructs were previously described (Upton et al., 2010). Retro- and lentiviral production, infection, and selection were previously described (Kaiser et al., 2008). siRNA transfections were performed with 200 pg of nontargeting, DAI, or RIP3 ON-TARGETplus SMARTpool siRNAs (Dharmacon) and Lipofectamine RNAiMAXX (Invitrogen) according to the manufacturer's recommendations, and assays were performed 48 hr posttransfection

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