Stealth siRNA(m) Stac3

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	C2C12 cells were plated in 24-well plates and grown to approximately 70% confluence. Cells in each well were transfected with Stac3 siRNAs (MSS239387, MSS239388, MSS239389 from Invitrogen, Carlsbad, CA), in combination of three (10 nM per siRNA) or separately (30 nM). Control cells were transfected with 30 nM scrambled siRNA (Invitrogen). The transfection reagent was Lipofectamine 2000 (Invitrogen). Since an appropriate Stac3 antibody was not available, knockdown efficiency was estimated by quantitative RT-PCR of Stac3 mRNA.

Protocol tips

C2C12 cells were plated in 24-well plates and grown to approximately 70% confluence. Cells in each well were transfected with Stac3 siRNAs (MSS239387, MSS239388, MSS239389 from Invitrogen, Carlsbad, CA), in combination of three (10 nM per siRNA) or separately (30 nM). Control cells were transfected with 30 nM scrambled siRNA (Invitrogen). The transfection reagent was Lipofectamine 2000 (Invitrogen). Since an appropriate Stac3 antibody was not available, knockdown efficiency was estimated by quantitative RT-PCR of Stac3 mRNA.

Publication protocol

C2C12 cells were plated in 24-well plates and grown to approximately 70% confluence. Cells in each well were transfected with Stac3 siRNAs (MSS239387, MSS239388, MSS239389 from Invitrogen, Carlsbad, CA), in combination of three (10 nM per siRNA) or separately (30 nM). Control cells were transfected with 30 nM scrambled siRNA (Invitrogen). The transfection reagent was Lipofectamine 2000 (Invitrogen). Since an appropriate Stac3 antibody was not available, knockdown efficiency was estimated by quantitative RT-PCR of Stac3 mRNA.

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