ON-TARGETplus Mouse Cd84 (12523) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	CD84 was done by siRNA introduced by electroporation using a Nepagene (Ichikawa, Chiba, Japan) Super Electroporator NEPA21 Type II. Adherent M201B4 cells (0.625 × 105) were electroporated in with 120 nM siRNA using the Adherent Cell Electrode with 125 V, 3 ms. CLL cells were electroporated using the Nepagene system using cuvettes (2mm gap), with 100 nM siRNA at 125 V, 3 ms. siRNA was purchased from GE Lifescience: mouse CD84: Dharmacon ON-TARGETplus SMARTpool mouse CD84 (Cat# L-048992-01); human CD84: Dharmacon ON-TARGETplus SMARTpool human CD84 (Cat# L-012623-00); non-specific control siRNA: Dharmacon ON-TARGETplus Non-targeting Pool (D-001810-10) (Little Chalfont, UK).

Protocol tips

CD84 was done by siRNA introduced by electroporation using a Nepagene (Ichikawa, Chiba, Japan) Super Electroporator NEPA21 Type II. Adherent M201B4 cells (0.625 × 105) were electroporated in with 120 nM siRNA using the Adherent Cell Electrode with 125 V, 3 ms. CLL cells were electroporated using the Nepagene system using cuvettes (2mm gap), with 100 nM siRNA at 125 V, 3 ms. siRNA was purchased from GE Lifescience: mouse CD84: Dharmacon ON-TARGETplus SMARTpool mouse CD84 (Cat# L-048992-01); human CD84: Dharmacon ON-TARGETplus SMARTpool human CD84 (Cat# L-012623-00); non-specific control siRNA: Dharmacon ON-TARGETplus Non-targeting Pool (D-001810-10) (Little Chalfont, UK).

Publication protocol

CD84 was done by siRNA introduced by electroporation using a Nepagene (Ichikawa, Chiba, Japan) Super Electroporator NEPA21 Type II. Adherent M201B4 cells (0.625 × 105) were electroporated in with 120 nM siRNA using the Adherent Cell Electrode with 125 V, 3 ms. CLL cells were electroporated using the Nepagene system using cuvettes (2mm gap), with 100 nM siRNA at 125 V, 3 ms. siRNA was purchased from GE Lifescience: mouse CD84: Dharmacon ON-TARGETplus SMARTpool mouse CD84 (Cat# L-048992-01); human CD84: Dharmacon ON-TARGETplus SMARTpool human CD84 (Cat# L-012623-00); non-specific control siRNA: Dharmacon ON-TARGETplus Non-targeting Pool (D-001810-10) (Little Chalfont, UK).

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