ON-TARGETplus Mouse Mapk14 (26416) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Monolayer cells were transfected with PKCδ siRNA expression plasmid pKD‐PKCδ‐v3 or pKD‐NegCon‐v3 (Upstate Biotechnology), ON‐TARGET smart pool siRNA, and ON‐TARGET plus siCONTROL Nontargeting Pool (Dharmacon, Lafayette, CO, USA) using Lipofectamine 2000 (Invitrogen). For p38 and ERK1/2 inhibition, cells were transfected with ON‐TARGET smart pool MAPK1 (ERK2) and p38 siRNA duplexes or nonspecific control siRNA duplexes (Dharmacon). Immunoblot analyses showed that expression of PKCδ, ERK1/2, and p38 remained low but detectable, whereas expression of β‐actin was unaffected by siRNA treatment.

Protocol tips

Monolayer cells were transfected with PKCδ siRNA expression plasmid pKD‐PKCδ‐v3 or pKD‐NegCon‐v3 (Upstate Biotechnology), ON‐TARGET smart pool siRNA, and ON‐TARGET plus siCONTROL Nontargeting Pool (Dharmacon, Lafayette, CO, USA) using Lipofectamine 2000 (Invitrogen). For p38 and ERK1/2 inhibition, cells were transfected with ON‐TARGET smart pool MAPK1 (ERK2) and p38 siRNA duplexes or nonspecific control siRNA duplexes (Dharmacon). Immunoblot analyses showed that expression of PKCδ, ERK1/2, and p38 remained low but detectable, whereas expression of β‐actin was unaffected by siRNA treatment.

Publication protocol

Monolayer cells were transfected with PKCδ siRNA expression plasmid pKD‐PKCδ‐v3 or pKD‐NegCon‐v3 (Upstate Biotechnology), ON‐TARGET smart pool siRNA, and ON‐TARGET plus siCONTROL Nontargeting Pool (Dharmacon, Lafayette, CO, USA) using Lipofectamine 2000 (Invitrogen). For p38 and ERK1/2 inhibition, cells were transfected with ON‐TARGET smart pool MAPK1 (ERK2) and p38 siRNA duplexes or nonspecific control siRNA duplexes (Dharmacon). Immunoblot analyses showed that expression of PKCδ, ERK1/2, and p38 remained low but detectable, whereas expression of β‐actin was unaffected by siRNA treatment.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Mouse - MC3T3-E1 p38/Mapk14 using ON-TARGETplus Mouse Mapk14 (26416) siRNA - SMARTpool from Horizon Discovery Ltd..

Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Mouse Mapk14 (26416) siRNA - SMARTpool below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Mouse - MC3T3-E1 p38/Mapk14 using ON-TARGETplus Mouse Mapk14 (26416) siRNA - SMARTpool from Horizon Discovery Ltd.. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.