ON-TARGETplus Mouse Mapk14 (26416) siRNA - SMARTpool

siRNA / miRNA gene silencing Mouse - MC3T3-E1 p38/Mapk14

Experiment
siRNA / miRNA gene silencing Mouse - MC3T3-E1 p38/Mapk14
Product
ON-TARGETplus Mouse Mapk14 (26416) siRNA - SMARTpool from Horizon Discovery Ltd.
Manufacturer
Horizon Discovery Ltd.

Protocol tips

Protocol tips
Monolayer cells were transfected with PKCδ siRNA expression plasmid pKD‐PKCδ‐v3 or pKD‐NegCon‐v3 (Upstate Biotechnology), ON‐TARGET smart pool siRNA, and ON‐TARGET plus siCONTROL Nontargeting Pool (Dharmacon, Lafayette, CO, USA) using Lipofectamine 2000 (Invitrogen). For p38 and ERK1/2 inhibition, cells were transfected with ON‐TARGET smart pool MAPK1 (ERK2) and p38 siRNA duplexes or nonspecific control siRNA duplexes (Dharmacon). Immunoblot analyses showed that expression of PKCδ, ERK1/2, and p38 remained low but detectable, whereas expression of β‐actin was unaffected by siRNA treatment.

Publication protocol

Monolayer cells were transfected with PKCδ siRNA expression plasmid pKD‐PKCδ‐v3 or pKD‐NegCon‐v3 (Upstate Biotechnology), ON‐TARGET smart pool siRNA, and ON‐TARGET plus siCONTROL Nontargeting Pool (Dharmacon, Lafayette, CO, USA) using Lipofectamine 2000 (Invitrogen). For p38 and ERK1/2 inhibition, cells were transfected with ON‐TARGET smart pool MAPK1 (ERK2) and p38 siRNA duplexes or nonspecific control siRNA duplexes (Dharmacon). Immunoblot analyses showed that expression of PKCδ, ERK1/2, and p38 remained low but detectable, whereas expression of β‐actin was unaffected by siRNA treatment.

Full paper   Login or join for free to view the full paper.

Reviews

ON-TARGETplus Mouse Mapk14 (26416) siRNA - SMARTpool from Horizon Discovery Ltd. has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Mouse - MC3T3-E1 p38/Mapk14 using ON-TARGETplus Mouse Mapk14 (26416) siRNA - SMARTpool from Horizon Discovery Ltd..

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Mouse Mapk14 (26416) siRNA - SMARTpool below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Mouse - MC3T3-E1 p38/Mapk14 using ON-TARGETplus Mouse Mapk14 (26416) siRNA - SMARTpool from Horizon Discovery Ltd.. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms