ON-TARGETplus Mouse Mapk1 (26413) siRNA - SMARTpool

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	Monolayer cells were transfected with PKCδ siRNA expression plasmid pKD‐PKCδ‐v3 or pKD‐NegCon‐v3 (Upstate Biotechnology), ON‐TARGET smart pool siRNA, and ON‐TARGET plus siCONTROL Nontargeting Pool (Dharmacon, Lafayette, CO, USA) using Lipofectamine 2000 (Invitrogen). For p38 and ERK1/2 inhibition, cells were transfected with ON‐TARGET smart pool MAPK1 (ERK2) and p38 siRNA duplexes or nonspecific control siRNA duplexes (Dharmacon). Immunoblot analyses showed that expression of PKCδ, ERK1/2, and p38 remained low but detectable, whereas expression of β‐actin was unaffected by siRNA treatment.

Protocol tips

Monolayer cells were transfected with PKCδ siRNA expression plasmid pKD‐PKCδ‐v3 or pKD‐NegCon‐v3 (Upstate Biotechnology), ON‐TARGET smart pool siRNA, and ON‐TARGET plus siCONTROL Nontargeting Pool (Dharmacon, Lafayette, CO, USA) using Lipofectamine 2000 (Invitrogen). For p38 and ERK1/2 inhibition, cells were transfected with ON‐TARGET smart pool MAPK1 (ERK2) and p38 siRNA duplexes or nonspecific control siRNA duplexes (Dharmacon). Immunoblot analyses showed that expression of PKCδ, ERK1/2, and p38 remained low but detectable, whereas expression of β‐actin was unaffected by siRNA treatment.

Publication protocol

Monolayer cells were transfected with PKCδ siRNA expression plasmid pKD‐PKCδ‐v3 or pKD‐NegCon‐v3 (Upstate Biotechnology), ON‐TARGET smart pool siRNA, and ON‐TARGET plus siCONTROL Nontargeting Pool (Dharmacon, Lafayette, CO, USA) using Lipofectamine 2000 (Invitrogen). For p38 and ERK1/2 inhibition, cells were transfected with ON‐TARGET smart pool MAPK1 (ERK2) and p38 siRNA duplexes or nonspecific control siRNA duplexes (Dharmacon). Immunoblot analyses showed that expression of PKCδ, ERK1/2, and p38 remained low but detectable, whereas expression of β‐actin was unaffected by siRNA treatment.

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