ON-TARGETplus Mouse Hspa5 (14828) siRNA - SMARTpool

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	The siRNAs against human calpain I, calpain II and control siRNA were purchased commercially from Santa Cruz Biotechnology. The ON-TARGET smart pool siRNA of GRP78 and scrambled siRNA were obtained from Dharmacon (Lafayette, CO). Cells were transfected with siRNAs (at a final concentration of 100 nM) using Lipofectamine 2000 (Invitrogen Life Technology) according to the manufacturer's instructions.

Protocol tips

The siRNAs against human calpain I, calpain II and control siRNA were purchased commercially from Santa Cruz Biotechnology. The ON-TARGET smart pool siRNA of GRP78 and scrambled siRNA were obtained from Dharmacon (Lafayette, CO). Cells were transfected with siRNAs (at a final concentration of 100 nM) using Lipofectamine 2000 (Invitrogen Life Technology) according to the manufacturer's instructions.

Publication protocol

The siRNAs against human calpain I, calpain II and control siRNA were purchased commercially from Santa Cruz Biotechnology. The ON-TARGET smart pool siRNA of GRP78 and scrambled siRNA were obtained from Dharmacon (Lafayette, CO). Cells were transfected with siRNAs (at a final concentration of 100 nM) using Lipofectamine 2000 (Invitrogen Life Technology) according to the manufacturer's instructions.

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