FITC Annexin V Apoptosis Detection Kit I (RUO)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Publication protocol

Cells were harvested and washed in ice-cold PBS. Then cells were incubated with Annexin V (PE Annexin V Apoptosis Detection Kit I, BD Biosciences, San Jose, CA, USA) for 15 min at 37°C in the dark and then incubated with propidium iodide (PI) for 10 min. The percentage of apoptotic cells was observed using the Elite ESP flow cytometer and data were analyzed with FlowJo 9.8.2 software.

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Manufacturer protocol

Download the product protocol from BD Biosciences for FITC Annexin V Apoptosis Detection Kit I (RUO) below.

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