siGENOME Mouse Amotl2 (56332) siRNA - SMARTpool

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	Cells were seeded on glass slides (BD Falcon Cultureslides BD Biosciences) coated with 1% BD Matrigel Basement Membrane Matrix (BD Biosciences) in growth medium without antibiotics. Next day the growth medium was exchanged for OPTI-MEM I Reduced Serum Media (Invitrogen) and siRNA transfections were performed using Oligofectamine Transfection Reagent (Invitrogen) according to the manufacturer’s protocol. Four hours after transfection serum was added to a final concentration of 20%. Cells were allowed to grow for 72 h prior to the evaluation by immunofluorescence staining or western blot. The following siRNA’s were used: siGENOME siRNAs against mouse AmotL2 (0.32 μM, M-062016–01, Dharmacon/Thermo Scientific). siGENOME siRNAs against mouse Par3 (0.64 μM, M-040036–01, Dharmacon/Thermo Scientific). non-targeting siRNA Pool #2 (0.32/ 0.64 μM, D 001206–14, Dharmacon/Thermo Scientific).siGENOME siRNAs against human AMOTL2 (0.32 μM, M-013232–00, Dharmacon/Thermo Scientific).ON-TARGETplus siRNAs against human PAR3 (0.32 μM, L-015602–00, Dharmacon/Thermo Scientific).

Protocol tips

Cells were seeded on glass slides (BD Falcon Cultureslides BD Biosciences) coated with 1% BD Matrigel Basement Membrane Matrix (BD Biosciences) in growth medium without antibiotics. Next day the growth medium was exchanged for OPTI-MEM I Reduced Serum Media (Invitrogen) and siRNA transfections were performed using Oligofectamine Transfection Reagent (Invitrogen) according to the manufacturer’s protocol. Four hours after transfection serum was added to a final concentration of 20%. Cells were allowed to grow for 72 h prior to the evaluation by immunofluorescence staining or western blot. The following siRNA’s were used: siGENOME siRNAs against mouse AmotL2 (0.32 μM, M-062016–01, Dharmacon/Thermo Scientific). siGENOME siRNAs against mouse Par3 (0.64 μM, M-040036–01, Dharmacon/Thermo Scientific). non-targeting siRNA Pool #2 (0.32/ 0.64 μM, D 001206–14, Dharmacon/Thermo Scientific).siGENOME siRNAs against human AMOTL2 (0.32 μM, M-013232–00, Dharmacon/Thermo Scientific).ON-TARGETplus siRNAs against human PAR3 (0.32 μM, L-015602–00, Dharmacon/Thermo Scientific).

Publication protocol

Cells were seeded on glass slides (BD Falcon Cultureslides BD Biosciences) coated with 1% BD Matrigel Basement Membrane Matrix (BD Biosciences) in growth medium without antibiotics. Next day the growth medium was exchanged for OPTI-MEM I Reduced Serum Media (Invitrogen) and siRNA transfections were performed using Oligofectamine Transfection Reagent (Invitrogen) according to the manufacturer’s protocol. Four hours after transfection serum was added to a final concentration of 20%. Cells were allowed to grow for 72 h prior to the evaluation by immunofluorescence staining or western blot.
The following siRNA’s were used: siGENOME siRNAs against mouse AmotL2 (0.32 μM, M-062016–01, Dharmacon/Thermo Scientific). siGENOME siRNAs against mouse Par3 (0.64 μM, M-040036–01, Dharmacon/Thermo Scientific). non-targeting siRNA Pool #2 (0.32/ 0.64 μM, D 001206–14, Dharmacon/Thermo Scientific).siGENOME siRNAs against human AMOTL2 (0.32 μM, M-013232–00, Dharmacon/Thermo Scientific).ON-TARGETplus siRNAs against human PAR3 (0.32 μM, L-015602–00, Dharmacon/Thermo Scientific).

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Download the product protocol from Horizon Discovery Ltd. for siGENOME Mouse Amotl2 (56332) siRNA - SMARTpool below.

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