Net siRNA (m)

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	To investigate the role of Elk-3 during EMT process of hepatocytes, specific small interference RNA (siRNA) targeting Elk-3 (siElk-3) and negative control siRNA (siCtrl) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). These siCtrl and siElk-3 were transfected into FL83B cells and AML12 cells using Lipofectamine™ 2000 transfection reagent (Invitrogen). At 48 hours post transfection, both cells were harvested and subjected to Western blot to determine whether Elk-3 affected EMT induced by TGF-β1.

Protocol tips

To investigate the role of Elk-3 during EMT process of hepatocytes, specific small interference RNA (siRNA) targeting Elk-3 (siElk-3) and negative control siRNA (siCtrl) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). These siCtrl and siElk-3 were transfected into FL83B cells and AML12 cells using Lipofectamine™ 2000 transfection reagent (Invitrogen). At 48 hours post transfection, both cells were harvested and subjected to Western blot to determine whether Elk-3 affected EMT induced by TGF-β1.

Publication protocol

To investigate the role of Elk-3 during EMT process of hepatocytes, specific small interference RNA (siRNA) targeting Elk-3 (siElk-3) and negative control siRNA (siCtrl) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). These siCtrl and siElk-3 were transfected into FL83B cells and AML12 cells using Lipofectamine™ 2000 transfection reagent (Invitrogen). At 48 hours post transfection, both cells were harvested and subjected to Western blot to determine whether Elk-3 affected EMT induced by TGF-β1.

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